Journal: World Journal of Gastroenterology : WJG

Article Title: Comparative genomic study of gastric epithelial cells co-cultured with Helicobacter pylori

doi: 10.3748/wjg.v18.i48.7212

Figure Lengend Snippet: Differentially expressed genes with a fold change of > 2.0 or < 0.5 in GES-1 cells co-cultured with the most virulent gastric carcinoma-derived strain vs the least virulent chronic gastritis-derived strain

Article Snippet: Briefly, goat-anti-HDAC6 (1:100; Santa Cruz Biotechnology, Inc., Heidelberg, Germany) and rabbit-anti-TRAF1 (1:100; BIOSS, Beijing, China) were used as primary antibodies.

Techniques: DNA Synthesis, Transduction, Cell Differentiation

Journal: World Journal of Gastroenterology : WJG

Article Title: Comparative genomic study of gastric epithelial cells co-cultured with Helicobacter pylori

doi: 10.3748/wjg.v18.i48.7212

Figure Lengend Snippet: Most differentially expressed genes in GES-1 cells co-cultured with the most virulent gastric carcinoma-derived strain vs the least virulent chronic gastritis-derived strain

Article Snippet: Briefly, goat-anti-HDAC6 (1:100; Santa Cruz Biotechnology, Inc., Heidelberg, Germany) and rabbit-anti-TRAF1 (1:100; BIOSS, Beijing, China) were used as primary antibodies.

Techniques: Transduction, Histone Deacetylase Assay

Journal: World Journal of Gastroenterology : WJG

Article Title: Comparative genomic study of gastric epithelial cells co-cultured with Helicobacter pylori

doi: 10.3748/wjg.v18.i48.7212

Figure Lengend Snippet: Verification of HDAC6 and TRAF1 up-regulation in GES-1 cells in response to the most and least virulent Helicobacter pylori strains as determined by quantitative real-time polymerase chain reaction analysis. A: Representative quantitation of mRNA expression of GAPDH, HDAC6 and TRAF1 in GES-1 cells (a), GES-1 cells co-cultured with the CG-derived Helicobacter pylori (H. pylori) strain (b) and GES-1 cells co-cultured with the GC-derived H. pylori strain (c); B: Graphic analysis showing mRNA expression of HDAC6 and TRAF1 expressions in GES-1 cells in GES-1 cells, GES-1 cells co-cultured with the CG-derived H. pylori strain and GES-1 cells co-cultured with the GC-derived H. pylori strain. bP < 0.01 vs the control; dP < 0.01 vs cells co-cultured with the CG-derived H. pylori strain.

Article Snippet: Briefly, goat-anti-HDAC6 (1:100; Santa Cruz Biotechnology, Inc., Heidelberg, Germany) and rabbit-anti-TRAF1 (1:100; BIOSS, Beijing, China) were used as primary antibodies.

Techniques: Real-time Polymerase Chain Reaction, Quantitation Assay, Expressing, Cell Culture, Derivative Assay

Journal: World Journal of Gastroenterology : WJG

Article Title: Comparative genomic study of gastric epithelial cells co-cultured with Helicobacter pylori

doi: 10.3748/wjg.v18.i48.7212

Figure Lengend Snippet: Histology and immunohistochemistry (400 ×, scale bar = 50 μm) of HDAC6 and TRAF1 in gastric specimens of patients with chronic gastritis (CG, n = 23), intestinal metaplasia (IM, n = 30), dysplasia (n = 33), and gastric carcinoma (GC, n = 27).

Article Snippet: Briefly, goat-anti-HDAC6 (1:100; Santa Cruz Biotechnology, Inc., Heidelberg, Germany) and rabbit-anti-TRAF1 (1:100; BIOSS, Beijing, China) were used as primary antibodies.

Techniques: Immunohistochemistry

Journal: Oncology reports

Article Title: Overexpression of goosecoid homeobox is associated with chemoresistance and poor prognosis in ovarian carcinoma.

doi: 10.3892/or.2014.3203

Figure Lengend Snippet: Figure 3. Correlation of mRNA expression by qRT-PCR with clinicopathological parameters. (A) High expression of GSC (≥1.5-fold) was significantly associated with LN metastasis. (B) Low expression of TNFRSF10A (≤0.5-fold) and high expression of SNAI1 (≥1.5-fold) were significantly associated with distant metastasis. (C) Low expression of TRAF1 (≤0.5-fold) and high expression of E2F1 (≥2-fold), FOS (≥2-fold), TERT (≥1.5-fold) and GSC (≥1.5-fold) were significantly associated with advanced clinical stage (stages III and IV).

Article Snippet: Slides were incubated overnight at 4 ̊C with the following primary antibodies and working dilutions: TRAF1 (1:2,000; Novus Biologicals, Littleton, CO, USA), TERT (1:200; Novus Biologicals) and GSC (1:200; Abnova, Taipei, Taiwan).

Techniques: Expressing, Quantitative RT-PCR

Journal: Oncology reports

Article Title: Overexpression of goosecoid homeobox is associated with chemoresistance and poor prognosis in ovarian carcinoma.

doi: 10.3892/or.2014.3203

Figure Lengend Snippet: Figure 4. Kaplan-Meier overall survival curves. The downregulation group of TRAF1 (≤0.5-fold) and upregulation group (≥1.5-fold) of TERT, GSC, NOTCH1 and SNAI1 were associated with a significantly worse overall survival than the upregulation or downregulation group, respectively, in the ovarian serous carcinoma cases.

Article Snippet: Slides were incubated overnight at 4 ̊C with the following primary antibodies and working dilutions: TRAF1 (1:2,000; Novus Biologicals, Littleton, CO, USA), TERT (1:200; Novus Biologicals) and GSC (1:200; Abnova, Taipei, Taiwan).

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The MyD88-independent pathway is not mobilized in human neutrophils stimulated via TLR4.

doi: 10.4049/jimmunol.178.11.7344

Figure Lengend Snippet: FIGURE 7. Expression of TLR4 signaling components in activated neutrophils and monocytes. Whole cell extracts prepared from neutro- phils and monocytes, as indicated in Materials and Methods, were elec- trophoresed and then immunoblotted using Abs specific for TLR4, MyD88, TIRAP/MAL, TRAM, NAP1, TBK1, IKKe, TRAF1, SARM, and SHP-2. For TRIF and TRAF3 proteins, immunoprecipitation fol- lowed by immunoblotting is shown. Depicted data are representative of at least three independent experiments.

Article Snippet: Anti IRF-3 Abs were from Active Motif (catalog no. 39033) or Santa Cruz Biotechnology (catalog no. sc-9082); anti phospho-IRF3 Abs (Ser396) were donated by Dr. J. Hiscott (McGill University, Montrèal, Canada); antiTRIF (catalog no. 3173) and anti-SARM (sterile and HEAT-armadillo motif; catalog no. 3295) Abs were from ProSci; anti-TLR4 (catalog no. sc-10741), IKK (catalog no. sc-10760), TRAF3 (catalog no. sc-948), I B- (catalog no. sc-371), TRAF1 (catalog no. sc-1831), and SHP-2 (catalog no. sc-280) Abs were from Santa Cruz Biotechnology; anti TIRAP/ MAL and MyD88 Abs were from Alexis Biochemicals (catalog no. ALX210-366) and Stressgen Biotechnologies (catalog no. 510), respectively; TBK1 mAbs (mAbs) were from Imgenex (catalog no. IMG-139A), and rabbit polyclonal TBK1 Abs were a gift from Dr. S. McWirther (Harvard University, Cambridge, MA); anti-TRAM Abs were from Drs. S. Sacre and B. Foxwell (Imperial College of Science, London, U.K.) (19); antiphosphotyrosine STAT1 (catalog no. 9171), phospho-p38 MAPK (catalog no. 9211), and native p38 MAPK (catalog no. 9212) Abs were from Cell Signaling; anti- -tubulin (catalog no. T5293) and actin (catalog no. A5060) Abs were from Sigma-Aldrich.

Techniques: Expressing, Immunoprecipitation, Western Blot